The Comparison of Chromosome Analysis Result by Manual and Software Cytovision Image Analysis Using Simple G-Banding

Gatot Ciptadi, Muhammad Nur Ihsan, Sri Rahayu, Veronica Margareta Ani Nurgiartiningsih, Mudawamah Mudawamah, Ardyah Ramadhina Irsanti Putri


Chromosome analysis or karyotyping is one among powerful methods to characterize normal or abnormal genetic of animals. On the basis of the important chromosome abnormalities and their negative effect in the near future, chromosomal investigation of breeding bull especially for Artificial Insemination (AI) began in different countries. Chromosomal abnormalities are usually considered to be a plague and are to eliminate. In Indonesia, AI implementation in cattle have been started intensively, especially using imported bulls (bos Taurus) i.e. Limousine. A number of cattle breeds have been reported on of the 50 exotic breeds with the problem of 1/29 translocation, then the chromosome analysis to be important to execute. Method performed by collecting blood samples from first generation of crossing breed Madura Cattle vs. Bos Taurus. Sample was added to medium (Karyo MAX Gibco) then placed in CO2 incubator at 38°C. Colcichine was added after 70 hours and kept for 2–3 hours. Slides were prepared and dried then stained with Giemsa. Slides were examined under high phase-contrast microscope, chromosome analysis using cytovision software and manual analysis straightly captured under microscope then arranged. Result of both method of karyotyping may accepted for analysing method of abnormal/normal chromosome. It showed that the 2 N diploid number of chromosome was normal was 60, there were 58 autosomes and 2 sex chromosomes in all cattle observed. It was observed that all cattle tested in this research were normal categories.  The karyotype analysis of all cattle showed that the chromosomes of one cell and different individual each breed varied in size, shape and position of centromere. It was recommended to performed chromosomal investigation of breeding bulls using advanced sophisticated tools of analysis like cytovision image analysis of fluorescent technique. Manual method was recommended only for analysis of normal or abnormal number of chromosome.


bull; artificial insemination; cytogenetic; karyotype

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Ahmad, I., K. Javed and A. Sattar. 2004. Screening of breeding bull of different breeds through karyotyping. Pakistan Veterinaire Journal 24 (4): 190-192.

Anis, M., S. Ali , Z . Ahmad and M.A. Khan. 1990. Studies on the karyotipes of Sahiwal cattle. Pakistan Vet Journal 10 (2): 88-91.

Chamdi, A. N. 2005. Review: The Characteristics of Genetic Resource of Bali Cattle (Bos-bibos banteng) and the Alternative of It's Conservation Methods. Biodiversitas 6: 1 70-75.

De Luca, J. C., P. Sister, A. Prando, A. Baldo and G. Giovambattista. 2007. Absence of 1/29 translocation in aberdeen Angus Breed. J. analecta veterinaria. 27 (2) 25-27.

Gallagher, D. S., Jr. B.C. Lewis, M. De Donato, S. K. Davis, J. F. Taylor and J. F. Edwards. 1999. Autosomal Trisomy 20 (61,XX,+20) in malformed Bovive Fetus. Journal Vet. Pathol, 36: 448 – 451.

Gustavsson, Z. 1979. Distribution and effect of the 1/29 robertonian translocation in cattle. J. Dairy Science., 62 (5): 825-835.

Iannuzzi, L., Di Meo, G. P., and Perucatti, A. 1996. G- and R-banded prometaphase karyotypes in goat. Caryologia, 49: 267-277.

Innayah, F. H. 2011. Skripsi : Karakteristik Habitat Banteng (Bos javanicus d’Alton 1832) di Taman Nasional Meru Betiri, Jawa Timur. Departemen Konservasi Sumberdaya Hutan dan Ekowisata Fakultas Kehutanan, Institut Pertanian Bogor.

ISCNDA. 1989. International System for Cytogenetic Nomenclature of Domestic Animals, (eds D. Dr BERARDINO, H. HAYES, R. FRIES and S. LONG). - Cytogenet. Cell Genet. 53: 65-79.

Kosarcik, S., M. Kovacevic, M. Jovicin, M Zivkoz Balos and D. Kosarcik. 2006. Changes in Karyotype in domestic animals discovered on the rarms in Vojvodina and their influence on reproduction. J. Genetika, 39 (2) 121 – 128.

Miyake , Y. I. 1996. Study on Reproductive disorders related to chromosome abnormalilies in Domestic Animals. Journal Reproduction and Development, 43 (6): 111-118.

Munoz, M. G., D. Ocanto, M. L. Madriz, R. Medina and D Vera. 1994. Incidence of 1/29 translocation in venezuelan Creole bulls. J. Theriogenology., 41 (2): 379-372.

Schmutz S. M, J. S. Moker V. Pavlyshyn, B. Haugen and E. G. Clark. 1997. Fertility Effect of the 14; 20 Roberstonnian translocation in cattle. J. Theriogenology., 47 (4) : 815-823.

Yamanaka, H. 1977. The possibility of cattle chromosome classification, identified by G-Banding patterns. Jap Journal veterinaire Research 25: 52-58.



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