Fusarium Rot Biological Control of Citrus caused by Fusarium oxysporum

Ovilya Kusuma Minarma Dewi, Abdul Latief Abadi, Sri Widyaningsih

Abstract


Fusarium oxysporum is a pathogen that causes Fusarium rot disease on citrus plants. The F. oxysporum is a soil-borne pathogen whose fungicide is not effective against it and difficult to cure. Thus, the use of Trichoderma spp. as one of the most effective and well-known biocontrol agents against many plant diseases in agriculture is needed. To test Trichoderma capabilities on the specific pathogen, method of this study consisted of isolation and rejuvenation of F. oxysporum and Trichoderma, morphological identification of the fungus, in vitro test of Trichoderma antagonistic ability against F. oxysporum on PDA medium, and in vivo test conducted in a green house on Rough Lemon (RL) and Japansche Citroen (JC) rootstock seeds to calculate the total disease incidence using a formula. The result of in vitro test of this study shows that 3 Trichoderma isolates (TJ, TKH, and TST) were able to inhibit the growth of F. oxysporum on PDA medium by 65.56%, 62.99%, and 61.19%, respectively. While in vivo test shows that the treatment of TJ isolates on RL seeds and TKH on JC seeds shows lowest disease incidence percentage of 3.33%. Therefore, this study proves that Trichoderma can be used as a biocontrol agent in controlling Fusarium rot disease in citrus plants. However, further research is needed to detect which Trichoderma species TJ and TKH isolates contain.


Keywords


Fusarium oxysporum; Trichoderma spp.; In vitro; In vivo; Citrus

Full Text:

PDF

References


Adnan, M., Islam, W., Shabbir, A., Khan, K. A., Ghramh, H. A., Huang, Z., Chen, H. Y. H., & Lu, G. dong. (2019). Plant defense against fungal pathogens by antagonistic fungi with Trichoderma in focus. Microbial Pathogenesis, 129(January), 7–18. https://doi.org/10.1016/j.micpath.2019.01.042

Ahsen, M. A., Naqvi, S. A., Jaskani, M. J., Waseem, M., Khan, I. A., Hussnain, K., Mehmood, K., Kamran, M., & Khan, M. M. (2019). Evaluation of Exotic Citrus Rootstocks Against Fusarium Spp. Journal of Global Innovations in Agricultural and Social Sciences, 7(4), 151–156. https://doi.org/10.22194/jgiass/7.874

Larran, S., Santamarina Siurana, M. P., Roselló Caselles, J., Simón, M. R., & Perelló, A. (2020). In vitro antagonistic activity of trichoderma harzianum against Fusarium sudanense causing seedling blight and seed rot on wheat. ACS Omega, 5(36), 23276–23283. https://doi.org/10.1021/acsomega.0c03090

Lecomte, C., Alabouvette, C., Edel-Hermann, V., Robert, F., & Steinberg, C. (2016). Biological control of ornamental plant diseases caused by Fusarium oxysporum: A review. Biological Control, 101, 17–30. https://doi.org/10.1016/j.biocontrol.2016.06.004

Mazrou, Y. S. A., Makhlouf, A. H., Elseehy, M. M., Awad, M. F., & Hassan, M. M. (2020). Antagonistic activity and molecular characterization of biological control agent Trichoderma harzianum from Saudi Arabia. Egyptian Journal of Biological Pest Control, 30(1). https://doi.org/10.1186/s41938-020-0207-8

Mbarga, J. B., Begoude, B. A. D., Ambang, Z., Meboma, M., Kuate, J., Ewbank, W., & Hoopen, G. M. te. (2020). Field testing an oil-based Trichoderma asperellum formulation for the biological control of cacao black pod disease, caused by Phytophthora megakarya. Crop Protection, 132(November 2019), 105134. https://doi.org/10.1016/j.cropro.2020.105134

Montes Vergara, D. E., Barboza-García, A., & Pérez-Cordero, A. (2022). Antifungal and growth activity of strains of Trichoderma spp. against the Avocado “tristeza” disease, Phytophthora cinnamomi. Egyptian Journal of Biological Pest Control, 32(1). https://doi.org/10.1186/s41938-022-00613-8

Nugroho, Y. A., Suharjono, S., & Widyaningsih, S. (2022). Biological control of citrus canker pathogen Xanthomonas citri subsp. citri using Rangpur lime endophytic bacteria. Egyptian Journal of Biological Pest Control, 32(1). https://doi.org/10.1186/s41938-022-00561-3

Sri Hastuti, U., & Rahmawati, I. (2016). The Antagonism Mechanism Of Trichoderma spp. Towards Fusarium solani Mold. The Journal of Pure and Applied Chemistry Research, 5(3), 178–181. https://doi.org/10.21776/ub.jpacr.2016.005.03.260

Taufiq, E. (2012). Potensi Trichoderma spp. dalam Menekan Perkembangan Penyakit Busuk Pucuk Vanili di Pembibitan. Buletin RISTRI, 3(1), 49–56.http://balittri.litbang.pertanian.go.id

Tchameni, S. N., Sameza, M. L., O’donovan, A., Fokom, R., Mangaptche Ngonkeu, E. L., Wakam Nana, L., Etoa, F., & Nwaga, D. (2017). Antagonism of Trichoderma asperellum against Phytophthora megakarya and its potential to promote cacao growth and induce biochemical defence. Mycology, 8(2), 84–92. https://doi.org/10.1080/21501203.2017.1300199

Triasih, U., Nugroho, Y. A., & Widyaningsih, S. (2021). Antimicrobial Activity of Pseudomonas fluorescens and Bacillus subtilis on Different Dilution Concentrations Against Various Citrus Post-Harvest Pathogens. Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020), 14(Kobicinc 2020), 535–539. https://doi.org/10.2991/absr.k.210621.089

Venkataramanamma, K., Reddy, B. V. B., Jayalakshmi, R. S., Jayalakshmi, V., & Rajendran, L. (2022). Isolation, in vitro evaluation of Bacillus spp. against Fusarium oxysporum f.sp. ciceris and their growth promotion activity. Egyptian Journal of Biological Pest Control, 32(1). https://doi.org/10.1186/s41938-022-00618-3

Watanabe, T. (2002). Pictorial Atlas of Soil and Seed Fungi Morphologies of Cultured Fungi and Key to Species (Second Edi). CRC Press.

Zapata-Sarmiento, D. H., Palacios-Pala, E. F., Rodríguez-Hernández, A. A., Medina Melchor, D. L., Rodríguez-Monroy, M., & Sepúlveda-Jiménez, G. (2020). Trichoderma asperellum, a potential biological control agent of Stemphylium vesicarium, on onion (Allium cepa L.). Biological Control, 140, 104105. https://doi.org/10.1016/j.biocontrol.2019.104105

Zhang, F., Ge, H., Zhang, F., Guo, N., Wang, Y., Chen, L., Ji, X., & Li, C. (2016). Biocontrol potential of Trichoderma harzianum isolate T-aloe against Sclerotinia sclerotiorum in soybean. Plant Physiology and Biochemistry, 100, 64–74. https://doi.org/10.1016/j.plaphy.2015.12.017




DOI: https://doi.org/10.21776/ub.rjls.2022.009.02.5

Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.