Study on Total Protein and Amino Acid Profile of Milkfish (Chanos chanos) Meat and Its Product from Sidoarjo, East Java

Jeny Ernawati Tambunan, Eddy Suprayitno, Titik Dwi Sulistiyati, Mikchaell Alfanov Pardamean Panjaitan


Milkfish is one of the most cultivated fish species in Indonesia because these fish have a high tolerance to the various environmental conditions of tropical waters and are resistant to many pests and diseases. This preliminary research was conducted to analyze the amino acid composition contained in milkfish meat and its product. A total of 20 fresh milkfish were obtained from ponds in Sidoarjo, East Java, in September 2020. Amino acid composition and protein content were analyzed. Based on its protein content, milkfish has been classified as a source of high protein. The amino acid profile of milkfish consists of approximately 17 types of amino acids. Glutamic acid, the amino acid present in the highest concentration, makes milkfish very popular.


Milkfish; Cooked Milkfish; Protein; Amino Acid

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Clandinin, M., Foxwell, A., Goh, Y. K., Layne, K., & Jumpsen, J. A. (1997). Omega-3 fatty acid intake results in a relationship between the fatty acid composition of LDL cholesterol ester and LDL cholesterol content in humans. Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1346(3), 247–252.

Demeester, J., Bracke, M., Vochten, R., & Lauwers, A. (1978). Differential Spectrophotometric Determination of Tyrosine and Tryptophan in Pharmaceutical Amino Acid Solutions. Journal of Pharmaceutical Sciences, 67(5), 729–730.

Fadjar, M., Islamy, R. A., & Herawati, E. Y. (2019). Short communication: First record of Arapaima gigas (Schinz, 1822) (Teleostei: Osteoglossomorpha), in the Brantas River, Sidoarjo, East Java, Indonesia. Biodiversitas Journal of Biological Diversity, 20(12), 3527–3531.

FAO. (2016). In : The State of World Fisheries and Aquaculture. Contributing to Food Security and Nutrition for All. From [Retrieved July 21 2020]

Hafiludin, H. (2015). The Analysis of Nutritional Content of Milkfishes Which Come from Different Habitats. Jurnal Kelautan, 8(1), 37–43.

Hasan, V., Widodo, M. S., Islamy, R. A., & Pebriani, D. A. A. (2020). New records of alligator gar, atractosteus spatula (Actinopterygii: Lepisosteiformes: Lepisosteidae) from bali and java, indonesia. Acta Ichthyologica et Piscatoria, 50(2).

Insani, L., Hasan, V., Valen, F. S., Pratama, F. S., Widodo, M. S., Faqih, A. R., Islamy, R. A., Mukti, A. T., & Isroni, W. (2020). Presence of the invasive nile tilapia Oreochromis niloticus linnaeus, 1758 (Perciformes, cichlidae) in the Yamdena Island, Indonesia. Ecology, Environment and Conservation, 26(3), 1115-1118.

Ishida, Y., Fujita, T., & Asai, K. (1981). New detection and separation method for amino acids by high-performance liquid chromatography. Journal of Chromatography A, 204, 143–148.

Islamy, R. A., & Hasan, V. (2020). Checklist of mangrove snails (Mollusca: Gastropoda) in South Coast of Pamekasan, Madura Island, East Java, Indonesia. Biodiversitas Journal of Biological Diversity, 21(7).

Malle, S., Tawali, A. B., Muhammad Tahir, M., & Bilang, M. (2019). Nutrient composition of milkfish (Chanos chanos, Forskal) from Pangkep, South Sulawesi, Indonesia. Malaysian Journal of Nutrition, 25(1), 155–162.

Ministry of Marine Affairs and Fisheries Republic of Indonesia. (2011). Kelautan dan perikanan dalam angka. Kementerian Kelautan dan Perikanan. Jakarta.

Pratama, W. W., Nursyam, H., Hariati, A. M., Islamy, R. A., & Hasan, V. (2020). Short communication: Proximate analysis, amino acid profile and albumin concentration of various weights of giant snakehead (channa micropeltes) from kapuas hulu, west kalimantan, Indonesia. Biodiversitas, 21(3).

Shiau, C. Y., Pong, Y. J., Chiou, T. K., & Chai, T. J. (1996). Free Amino Acids and Nucleotide-Related Compounds in Milkfish (Chanos chanos) Muscles and Viscera. Journal of Agricultural and Food Chemistry, 44(9), 2650–2653.

Shiau, C. Y., Pong, Y. J., Chiou, T. K., & Tin, Y. Y. (2001). Effect of starvation on free histidine and amino acids in white muscle of milkfish Chanos chanos. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 128(3), 501–506.

Sil Valen, F., Sri Widodo, M., Kilawati, Y., & Islamy, R. A. (2019). Phytogenetic Relationships of Mystacoleucus marginatus (Valenciennes 1842) based on Cytochrome Oxidase C Subunit I (COI) Gene. Research Journal of Life Science, 6(1), 19-28.

WHO. (2007). Protein and amino acid requirements in human nutrition Report of a joint FAO/WHO/UNU expert consultation (WHO Technical Report Series 935). World Health Organization, Geneva, Switzerland.



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