Morphological Characteristics of Bioactive Compounds on Api-Api Mangrove Leaves Extract (Avicennia marina) Based on Leaves Age

Muhamad Zaky Zamani, Arief Prajitno, Mohamad Fadjar

Abstract


Api-api leaves A. marina has a variety of active compounds that have been confirmed to too wordy be used as antibacterial agents, where harvesting of mangrove leaves is carried out at the location of Betoyoguci Gresik and differentiated by age, this age differentiation is based on morphological characteristics because it has color differences significant. Where age is one of the factors that affect the level of active compounds found in leaves. Extraction results of mangrove api-api gave different yield levels in each age level. The order of yields from the largest to the smallest is the old leaves 3%, fall 2.86% and young leaves 2.04%. Whereas based on phytochemical test the best extracts were obtained, namely old leaves, fall leaves and young leaves, FT-IR results with 10 detected absorption files which indicate the presence of functional group characteristics that can be used as an illustration of the presence of antibacterial compounds. In the LC-MS test with identification of several molecular weights which indicated a signal derivative of flavonoid compounds in the form of dihydroquercetin, quercetin-3-O-β-D-xylopyranoside, quercetin-3-O-β-D-galactopyranoside, isohametin-7-O-pentoside and routine, and derivatives of detected alkaloid compounds in the form of berberine and papaverine.

Keywords


Avicennia marina; Leaves Age; Antibacterial Content

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References


Arima, Hidetoshi, Hitoshi Ashida, and Genichi Danno. 2002. Rutin-Enhanced Antibacterial Activities of Flavonoids against Bacillus cereus and Salmonella enteritidis. Bioscience, Biotechnology and Biochemistry, 66(5): 1009–1014.

Bandyopadhyay, Samiran, Pabitra H Patra, Achintya Mahanti, and Dipak K. Mondal. 2015. Potential Antibacterial Activity of Berberine against Multi Drug Resistant Enterovirulent Escherichia coli Isolated from Yaks (Poephagus Grunniens) with Haemorrhagic Diarrhoea. Asian Pacific Journal of Tropical Medicine, 315–319.

Battu, Sunil Kumar, M. A. Repka., S. Maddineni., A. G. Chittiboyina., M. A. Avery and S. Majumdar. 2010. Physicochemical Characterization of Berberine Chloride : A Perspective in the Development of a Solution Dosage Form for Oral Delivery Physicochemical Characterization of Berberine Chloride : A Perspective in the Development of a Solution Dosage Form for O, 11(3): 1466–1475.

Bruno, Fernando F., and J. Kumar A. Trotta, S. Fossey, S. Nagarajan, R. Nagarajan, L. A. Samuelson. 2010. Enzymatic Synthesis and Characterization of PolyQuercetin. Journal of Macromolecular Science, Part A: Pure and Applied Chemistry, 47: 1191–1196.

Dabas, Deepti, Ryan J Elias, Gregory R Ziegler, and Joshua D Lambert. 2019. In Vitro Antioxidant and Cancer Inhibitory Activity of a Colored Avocado Seed Extract. International Journal of Food Science, 1-7.

Dhayanithi, N. B., T. T. A. Kumar, J. Arockiaraj, C. Balasundaram, R. Harikrishnan. 2015. Fish & Shell Fish Immunology Dietary Supplementation of Avicennia marina Extract on Immune Protection and Disease Resistance in Amphiprion Sebae against Vibrio alginolyticus. Fish and Shellfish Immunology, 45(1): 52–58.

Ekawaty, J. Mei, H. Nursyam, and Hardoko. 2015. Characterization of Avicennia marina Leaves Fraction as Antibacterial of Aeromonas salmonicida. Journal of Life Science and Biomedicine, 5(5): 149–152.

Haditomo, A. H. J., A. M. Lusiastuti dan Widanarni. 2016. The Study of Bacillus Firmus as Probiotic Candidate in Supressing Aeromonas hydrophila in Culture Media. Indonesian Journal of Fisheries Science and Technology, 11(2): 111–114.

Harizon., Pujiastuti, B., Kurnia, D., Sumiarsa, D dan Shiono, Y. 2014. Triterpenoid Lupan Dari Kulit Batang Sonneratia alba (Lythraceae). Bionatura, Jurnal Ilmu-ilmu Hayati dan Fisik, 16(1): 25–29.

Jain, P. K, Anjali Soni, Preeti Jain, and Jeetendra Bhawsar. 2016. Phytochemical Analysis of Mentha Spicata Plant Extract Using UV-VIS, FTIR and GC / MS Technique. Journal of Chemical and Pharmaceutical Research, 8(2): 1–6.

Jaisinghani, Renu Narendra. 2017. Antibacterial Properties of Quercetin. Microbiology Research, 8(6877): 13–14.

Karimuna, Siti Rahmah. 2015. Uji Korelasi Konsentrasi Hara n, p, Dan k Daun Dengan Produksi Senyawa Bioaktif Kemuning (Murraya paniculata (l.) Jack) Akibat Aplikasi Pupuk Kandang Ayam.[Tesis]. 73p.

Kumar, G. D., M. Karthik, R. Rajakumar, and K. Baskar. 2018. Effects of Avicennia marina Extracts on Labeo rohita (Ham) Challenged with Pseudomonas fluorescens. Biotechnology Research and Innovation, 1-6.

Ling, Yao., W. Ling, L. Qian, H. Mei, Z. Shu-Yuan, L. Kang, J. Jian-Qin. 2018. Novel Berberine Derivatives : Design , Synthesis , Antimicrobial Effects, and Molecular Docking Studies. Chinese Journal of Natural Medicines, 16(10): 774–781.

Maalik, Aneela., F. A. Khan., A. Mumtaz., A. Mehmood., S. Azhar., M. Atif., S. Karim., Y. Altaf and I. Tariq. 2014. Pharmacological Applications of Quercetin and Its Derivatives: A Short Review. Tropical Journal of Pharmaceutical Research, 13(9): 1561–1566.

Mallikarjuna, K., B. Deva Prasad Raju G. Narasimha, G. R. Dillip, B. Praveen, B. Shreedhar, C. S. Lakshmi, B. V. S. Reddy. 2011. Green Synthesis Of Silver Nanoparticles Using Ocimum Leaf Extract And Their Characterization. Digest Journal of Nanomaterials and Biostructures, 6(1): 181–186.

Ningrum, Dita W, and E. Fachriyah D. Kusrini. 2017. Uji Aktivitas Antioksidan Senyawa Flavonoid Dari Ekstrak Etanol Daun Johar (Senna siamea Lamk). Jurnal Kimia Sains dan Aplikasi, 20(3): 123–129.

Permata, D. A and A. Asben. 2017. Karakteristik Dan Senyawa Bioaktif Ekstrak Kering Daun Kluwih Dari Posisi Daun Yang Berbeda. Jurnal Teknologi Pertanian Andalas, 21(2): 79–85.

Rachmawati, S and Widiyanti P. M. 2013. Kadar Melamin Pada Produk Berbahan Susu Dan Susu Bubuk Yang Dianalisis Secara Liquid Chromatography Mass Spectrometry ( LC-MS ). JITV, 18(1): 63–69.

Rohaeti, E., R. Heryanto, M. Rafi, A. Wahyuningrum, and L. K. Darusman. 2011. Prediksi Kadar Flavonoid Total Tempuyung (Sonchus arvensis L.) Menggunakan Kombinasi Spektroskopi Ir Dengan Regresi Kuadrat Terkecil Parsial. Jurnal Kimia, 2(5): 101–108.

Schimel, J. P and S. Hattenschwiler. 2007. Nitrogen Transfer between Decomposing Leaves of Different N Status. Soil Biology & Biochemistry, 39: 1428–1436.

Setyowati, E., S. B. Prayitnoand Sarjito. 2014. Pengaruh Perendaman Ekstrak Daun Jambu Biji (Psidium guajava. L) Terhadap Kelulushidupan dan Histologi Hati Ikan Patin (Pangasius hypophtalamus) yang Diinfeksi Bakteri Edwardsiella tarda. Journal of Aquaculture Management and Technology, 3: 44–52.

Sopiah, Nida. 2014. Aktivasi Jerami Padi Sebagai Oil Sorbent Menggunakan Asam Asetat. Jurnal Teknologi Lingkungan, 27–30.

Syafitri, N. E., M. Bintang, and S. Falah. 2014. Kandungan Fitokimia, Total Fenol, Dan Total Flavonoid Ekstrak Buah. Current Biochemistry, 1(3): 105–115.

Ulmursida, Anisa, A. Ambariyanto, and A. Trianto. 2017. Antibacterial Activity of Mangrove Avicennia marina Leaves Extract against Virgibacillus marismortui and Micrococcus luteus Bacteria. AACL Bioflux, 10(2): 372–380.

Xie, Yixi, and X. Chen and L. Ren W. Yang, F. Tang. 2015. Antibacterial Activities of Flavonoids : Structure- Activity Relationship and Mechanism. Current Medicinal Chemistry, 22: 132–149.




DOI: https://doi.org/10.21776/ub.rjls.2019.006.03.4

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