Phytochemical analysis, FTIR fingerprint and bioactivity of crude extract and fractions of Mesua ferrea L.

Dwi Hartanti, Zainur Rahman Hakim, Anna Nurlativah, Arinda Nur Cahyani, Fadhilah Zahra Nadia, Asmiyenti Djaliasrin Djalil


Mesua ferrea L. (Ceylon ironwood, Calophyllaceae) is a popular medicinal plant with a long history in South and Southeast Asia folk medicines. In this study, we report the phytochemical analysis, FTIR fingerprint, as well as antimicrobial and antioxidant activities of the crude extract of leaves of M. ferrea and its fractions. The dried leaves of M. ferrea were extracted with ethanol by the re-maceration method. The crude extract was further partitioned in ethanol-water and ethyl acetate to obtain ethanol and ethyl acetate fractions, respectively. The identification of compounds in the extract and fractions was conducted according to the standard phytochemical screening method. Fourier-transform infrared spectroscopy (FTIR) was utilized to record their metabolites fingerprint. The antimicrobial activity was evaluated with the disk diffusion method against Escherichia coli, Bacillus subtilis, Candida albicans, and Saccharomyces cerevisiae. The antioxidant activity assay was conducted with 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging method. The crude extract of M. ferrea leaves and it fractions contained saponins, phenolic compounds, flavonoids, and terpenoids. The FTIR data supported the presence of terpenoids and glycosides in those samples as well. The extract and fractions demonstrated considerable antimicrobial activity against all tested bacteria and fungi, with MIC values ranged 3.9-31.3 μg/ml.  The ethyl acetate fraction of M. ferrea leaves showed a moderate antioxidant activity with an IC50 value of 49.19 μg/ml. 


bioactivities, extract, fractions, Mesua ferrea L., secondary metabolites


Asif, M., Shafaei, A., Jafari, S. F., Mohamed, S. K., Ezzat, M. O., Majid, A. S. A., … Majid, A. M. S. A. (2016). Isoledene from Mesua ferrea oleo-gum resin induces apoptosis in HCT 116 cells through ROS-mediated modulation of multiple proteins in the apoptotic pathways: A mechanistic study. Toxicology Letters, 257, 84–96.

Chahar, M. K., Kumar, D. S. S., Lokesh, T., & Manohara, K. P. (2012). In-vivo antioxidant and immunomodulatory activity of mesuol isolated from Mesua ferrea L. seed oil. International Imunopharmacology, 12(4), 386–391.

Chaitanya, K. K., Rao, K. K., Sastry, Y. N., Padal, S. B., Lakshmi, A. R., & Rao, D. G. (2015). Anti-inflammatory, antioxidant and phytochemical analysis of Mesua ferrea bark extracts. International Journal of Current Trends in Pharmaceutical Research, 3(3), 891–902.

Chaithanya, K. K., Gopalakrishnan, V. K., Hagos, Z., & Rao, D. G. (2018). Mesuaferrin A, bioactive flavonoid isolated from the bark of Mesua ferrea L. against phospholipase A2, cyclooxygenase and lipoxygenase: An in vitro, in vivo and in silico approach. International Journal of Pharmacy and Pharmaceutical Sciences, 10(2), 102–106.

Chanda, S., Rakholiya, K., & Parekh, J. (2013). Indian medicinal herb: Antimicrobial efficacy of Mesua ferrea L. seed extracted in different solvents against infection causing pathogenic strains. Journal of Acute Disease, 2(4), 277–281.

Chukaew, A., Saithong, S., Chusri, S., Limsuwan, S., Watanapokasin, R., Voravuthikunchai, S. P., & Chakthong, S. (2019). Cytotoxic xanthones from the roots of Mesua ferrea L. Phytochemistry, 157, 64–70.

Garg, S., Sharma, K., Ranjan, R., & Attri, P. (2009). Evaluation of antioxidant and hepatoprotective efficacy of methanolic extract of Mesua ferrea Linn leaves in experimentally challenged mice. International Journal of PharmTech Research, 1(4), 1692–1696.

Hartanti, D., Arafani, Z. M., Nurlativah, A., & Hakim, Z. R. (2017). Constituents and antibacterial activity of extract of Nagasari (Mesua ferrea) leaves. In A. D. Djalil, P. I. Utami, D. Hartanti, G. F. Galistiani, & W. Utaminingrum (Eds.), Proceeding of The 2nd UMP-PIC & 8th ISCC (pp. 9–15). Purwokerto: Universitas Muhammadiyah Purwokerto.

Hassan, M. T., Ali, M. S., Alimuzzaman, M., & Raihan, S. Z. (2006). Analgesic activity of Mesua ferrea Linn. Dhaka University Journal of Pharmaceutical Sciences, 5(1–2), 73–75.

Jalalpure, S. S., Mandavkar, Y. D., Khalure, P. R., Shinde, G. S., Shelar, P. A., & Shah, A. S. (2011). Antiarthritic activity of various extracts of Mesua ferrea Linn. seed. Journal of Ethnopharmacology, 138(3), 700–704.

Jaradat, N., Hussen, F., & Ali, A. Al. (2015). Preliminary phytochemical screening, quantitative estimation of total flavonoids, total phenols and antioxidant activity of Ephedra alata. Journal of Material and Environmental Sciences, 6(6), 1771–1778.

Keawsaard, S., & Kongtaweelert, S. (2012). Antioxidant, antibacterial, anticancer activities and chemical constituents of the essential oil from Mesua ferrea leaves. Chiang Mai Journal of Science, 39(3), 455–463.

Kumar, V. P., Chauhan, N. S., Padh, H., & Rajani, M. (2006). Search for antibacterial and antifungal agents from selected Indian medicinal plants. Journal of Ethnopharmacology, 107, 182–188.

Mazumder, R., Dastidar, S. G., Basu, S. P., Mazumder, A., & Singh, S. K. (2004). Antibacterial potentiality of Mesua ferrea Linn. flowers. Phytotherapy Research, 18(10), 824–826.

Permatasari, L., & Rohman, A. (2017). 2,2’-diphenil-1-picrylhydrazil (DPPH) radical scavenging activity of extracts and fractions of Rambutan (Nephelium lappaceum L.) peel. Research Journal of Phytochemistry, 10, 75–80. 10.3923/rjphyto.2016.75.80

Phuong, N. T. D., & Nhat, D. M. (2015). Antimicrobial activities of the extracts from bark of ironwood (Mesua ferrea) and their role in production of traditional sugar palm wine. Journal of Food and Nutrition Sciences, 3(1), 65–69.

Rajesh, K. P., Manjunatha, H., Krishna, V., & Swamy, B. E. K. (2013). Potential in vitro antioxidant and protective effects of Mesua ferrea Linn. bark extracts on induced oxidative damage. Industrial Crops and Products, 47, 186– 198.

Rawat, V., & Upadhyaya, K. (2013). Evaluation of antimicrobial activity and preliminary phytochemical screening of Mesua ferrea seed extract. Journal of Natural Products, 6, 17–26.

Roy, S. K., Kumari, N., Pahwa, S., Agrahari, U. C., Bhutani, K. K., Jachak, S. M., & Nandanwar, H. (2013). NorA efflux pump inhibitory activity of coumarins from Mesua ferrea. Fitoterapia, 90, 140–150.

Shahraki, S., Khajavirad, A., Shafei, M. N., Mahmoudi, M., & Tabasi, N. S. (2016). Effect of total hydroalcholic extract of Nigella sativa and its n-hexane and ethyl acetate fractions on ACHN and GP-293 cell lines. Journal of Traditional and Complementary Medicines, 6(6), 89–96.

Suparman, S., Diniatik, D., Kusumaningrum, D., & Yulianto, Y. (2012). Studi etnobotani tumbuhan sub kelas Rosidae dan penggunaannya sebagai obat tradisional di Kecamatan Baturraden Kabupaten Banyumas. Pharmacy, 8(2), 1–8.

Suresh, C., Chandra, T. R., Meeta, K., & Deep, K. G. (2014). Nagakesara (Mesua ferrea Linn.) - A gift for GIT, skin and bleeding disorder. International Journal of Ayurveda & Anternative Medicine, 2(4), 48–53.

Teh, S. S., Cheng, G., Ee, L., Rahmani, M., Taufiq-yap, Y. H., Go, R., & Mah, S. H. (2011). Pyranoxanthones from Mesua ferrea. Molecules, 16, 5647–5654.

Teh, S. S., Ee, G. C. L., Mah, S. H., Lim, Y. M., & Ahmad, Z. (2013). Cytotoxicity and structure-activity relationships of Xanthone derivatives from Mesua beccariana, Mesua ferrea and Mesua congestiflora towards nine human cancer cell lines. Molecules, 18(2), 1985–1994.

Topală, C. M., Tătaru, L. D., & Ducu, C. (2017). ATR-FTIR spectra fingerprinting of medicinal herbs extracts prepared using microwave extraction. Arabian Journal of Medicinal & Aromatic Plants, 3(1), 1–9.

Ullah, H. M. A., Tareq, S. M., Huq, I., Uddin, M. B., & Salauddin, M. (2013). Antimicrobial and cytotoxic activity assessment of the aqueous methanolic and pet-ether extract of the leaves of Mesua ferrea. International Journal of Pharmaceutical Science and Research, 4(9), 3563–3568.

Verotta, L., Lovaglio, E., Vidari, G., & Neric, G. (2004). 4-Alkyl- and 4-phenylcoumarins from Mesua ferrea as promising multidrug resistant antibacterials. Phytochemistry, 65(21), 2867–2879.

Viena, V., Yunita, I., Irhamni, Saudah, & Ernilasari. (2018). Biodiversitas tumbuhan obat oleh masyarakat Gampong Pulo Seunong Kecamatan Tangse Kabupaten Pidie. Elkawnie: Journal of Islamic Science and Technology, 4(1), 89–100.

Zavoi, S., Fetea, F., Ranga, F., Pop, R. M., Baciu, A., & Socaciu, C. (2011). Comparative fingerprint and extraction yield of medicinal herb phenolics with hepatoprotective potential, as determined by UV-Vis and FT-MIR spectroscopy. Notulae Botanicae Horti Agrobotanici, 39(2), 82–89.

Zhao, J., Avula, B., Chan, M., Clément, C., Kreuzer, M., & Khan, I. A. (2012). Metabolomic differentiation of Maca (Lepidium meyenii) accessions cultivated under different conditions using NMR and chemometric analysis. Planta Medica, 78(1), 90–101.



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