The Effect of Ulva lactuta Polysaccharide Extract on Total Haemocyte Count and Phagocytic Activity of L. vannamei

Suleman Suleman, Sri Andayani, Ating Yuniarti


This research aimed to evaluate the effect of polysaccharide extract of Ulva lactuta on the total haemocytes count (THC) and the phagocytic activities (PA) on Litopenaeus vannamei. The concentration of the extract used in this research was 5 ppm and it was applied through the immersion. The haemolymph was collected after the administration in the different periods at hour of 4 and 24. The results showed that the administration of polysaccharide extracts increased THC and PA in L. vannamei significantly (p<0.05). THC and PA of white-leg shrimp with polysaccharide extract was higher than that without the administration of the extract. The enhancement of THC was found after the administration at hour 4 from 5.00 cells. ml-1 to 5.26 x 104 cells. ml-1. However, the THC level decreased after 24 hours at the value of 5.19 x 104 cells. The increasing pattern was also true for PA of L. vannamei, from 40.44% to 53.67% (hour 4). At the hour of 24, the PA was found decreased to the level and then decreased 45.67 ±1.53. On the contrary, there were no enhancement of THC and PA in the treatment without polysaccharide extract of Ulva lactuta.


immunostimulant; green seaweed; white-leg shrimp

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Abd El-Baky, H. H., El Baz, F. K., El-Baroty, G. S. 2008. Evaluation of marine alga Ulva Lactuca L. as a source of natural preservative ingredient. Electron. J. Environ. Agric. Food Chem. 7, 3353–3367.

Abdollahi-Arpanahi, D., Soltani, E., Jafaryan, H., Soltani, M., Naderi-Samani, M., Campa-Córdova, A.I. 2018. Efficacy of two commercial and indigenous probiotics, Bacillus subtilis and Bacillus licheniformis on growth performance, immuno-physiology and resistance response of juvenile white shrimp (Litopenaeus vannamei). Aquaculture 496, 43–49.

Aguirre-Guzmán, G., Sánchez-Martínez, J. G., Campa-Córdova, A. I., Luna-González, A., Ascencio, F. 2009. Penaeid shrimp immune system. Thai J. Vet. Med.

Anderson, D. P. 1992. Immunostimulants, adjuvants, and vaccine carriers in fish: Applications to aquaculture. Annu. Rev. Fish Dis. 2, 281–307.

Bachère, E., Mialhe, E., Noël, D., Boulo, V., Morvan, A., Rodriguez, J. 1995. Knowledge and research prospects in marine mollusc and crustacean immunology. Aquaculture 132, 17–32.

Bellanti, J. A. 1989. Immunology III. Yogyakarta : Gadja Mada University Press.

Castro, R., Piazzon, M.C., Zarra, I., Leiro, J., Noya, M., Lamas, J. 2006. Stimulation of turbot phagocytes by Ulva rigida C. Agardh polysaccharides. Aquaculture 254, 9–20.

Castro, R., Zarra, I., Lamas, J. 2004. Water-soluble seaweed extracts modulate the respiratory burst activity of turbot phagocytes. Aquaculture 229, 67–78.

Chen, J. -C., Hou, W. 2005. The immunostimulatory effect of hot-water extract of Gracilaria tenuistipitata on the white shrimp Litopenaeus vannamei and its resistance against Vibrio alginolyticus. Fish Shellfish Immunol.

Cheng, W., Liu, C. -H., Tsai, C. -H., Chen, J. -C. 2005. Molecular cloning and characterisation of a pattern recognition molecule, lipopolysaccharide- and β-1,3-glucan binding protein (LGBP) from the white shrimp Litopenaeus vannamei. Fish Shellfish Immunol., 18, 297–310.

Chojnacka, K., Saeid, A., Witkowska, Z., Tuhy, L. 2012. Biologically active compounds in seaweed extracts-the prospects for the application. Open Conf. Proc. J. 3, 20–28.

Dugger, D. M, Jorry, D. E. 1999. Biomodulation of the non-specific immune response in marine shrimp with beta-glucan. Aquac. Mag. 1, 81–89.

Gannam, A., M. Schrock, R. 1999. Immunostimulants in Fish Diets, Journal of Applied Aquaculture.

Johansson, M. W., Keyser, P., Sritunyalucksana, K., Söderhäll, K. 2000. Crustacean haemocytes and haematopoiesis. Aquaculture 191, 45–52.

Johansson, M. W., Keyser, P., Sritunyalucksana, K., Söderhäll, K. 2000. Crustacean haemocytes and haematopoiesis. Aquaculture.

Johnny, F., Roza, D., Mahardika, K., Zafran, Z., Prijono, A. 2017. Penggunaan Immunostimulan Untuk Meningkatkan Kekebalan Non-Spesifik Benih Ikan Kerapu Lumpur (Ephinephelus coioides) Terhadap Infeksi Virus Irido, Jurnal Penelitian Perikanan Indonesia.

Peraza-Gómez, V., Luna-González, A., González-Prieto, J.M., Fierro-Coronado, A., González-Ocampo, H.A. 2014. Protective effect of microbial immunostimulants and antiviral plants against WSSV in Litopenaeus vannamei cultured under laboratory conditions. Aquaculture 420–421, 160–164.

Putri, F. M., Sarjito, Suminto. 2013. The Effect of Spirulina sp. Addition to Artificial Diet on The Total Haemocyte Count and Phagocytosis Activity of White Shrimp (Litopenaeus vannamei). J. Aquac. Manag. Technol. 2, 102–112.

Ridlo, A., Pramesti, R. 2012. Aplikasi Ekstrak Rumput Laut Sebagai Agen Imunostimulan Sistem Pertahanan Non Spesifik Pada Udang (Litopennaeus vannamei). ILMU Kelaut. Indones. J. … 14, 133–137.

Sakai, M. 1999. Current research status of fish immunostimulants. Aquaculture 172, 63–92.

Sakai, M. 1999. Current research status of fish immunostimulants. Aquaculture 172, 63–92.

Selvin, J., Huxley, A. J., Lipton, A. P. 2004. Immunomodulatory potential of marine secondary metabolites against bacterial diseases of shrimp. Aquaculture 230, 241–248.

Selvin, J., Manilal, A., Sujith, S., Seghal Kiran, G., Premnath Lipton, A. 2011. Efficacy of marine green alga Ulva fasciata extract on the management of shrimp bacterial diseases. Lat. Am. J. Aquat. Res. 39, 197–204.

Smith, V. J., Brown, J. H., Hauton, C. 2003. Immunostimulation in crustaceans: does it really protect against infection? Fish Shellfish Immunol. 15, 71–90.

Söderhäll, K., Cerenius, L. 1992. Crustacean immunity. Annu. Rev. Fish Dis.

Tabarsa, M., Lee, S. -J., You, S. 2012. Structural analysis of immunostimulating sulfated polysaccharides from Ulva pertusa. Carbohydr. Res., 361, 141–147.

Tabarsa, M., You, S. G., Dabaghian, E. H., Surayot, U. 2018. Water-soluble polysaccharides from Ulva intestinalis: Molecular properties, structural elucidation and immunomodulatory activities. J. Food Drug Anal. 26, 599–608.

Vargas-Albores, F., Guzmán, M. A., Ochoa, J. L. 1993. An anticoagulant solution for haemolymph collection and prophenoloxidase studies of penaeid shrimp (Penaeus californiensis). Comp. Biochem. Physiol. -- Part A Physiol.

Xian, J. A., Wang, A. L., Tian, J. X., Huang, J. W., Ye, C. X., Wang, W. N., Sun, R. Y. 2009. Morphologic, physiological and immunological changes of haemocytes from Litopenaeus vannamei treated by lipopolysaccharide. Aquaculture.

Yin, G., Jeney, G., Racz, T., Xu, P., Jun, X., Jeney, Z. 2006. Effect of two Chinese herbs (Astragalus radix and Scutellaria radix) on non-specific immune response of tilapia, Oreochromis niloticus. Aquaculture 253, 39–47.



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