Development of an Experimental Model of Metabolic Syndrome in Sprague Dawley Rat

Mohammad Saifur Rohman, Mifetika Lukitasari, Dwi Adi Nugroho, Widodo Nashi, Nur Ida Panca Nugraheini, Teguh Wahyu Sardjono

Abstract


The study was designed to establish an experimental models of metabolic syndrome that adequately mimic metabolic syndrome criteria as determined by NCEP ATP III.

Eighteen Male Sprague dawley rats, 2 - 3 months old were used in the study. Combination of high fat and high sucrose (HFHS) diet for eight weeks and streptozotocin (STZ) injection in the second week was administered to induce metabolic syndrome. The body weight and biochemical parameters (blood glucose, triglyceride, HDL-cholesterol) were measured every 2 weeks. The rats with blood glucose (>126mg/dL), triglyceride (TG) (>150mg/dL), high systolic blood pressure (≥140 mmHg), and reduced HDL levels (<40 mg/dL) confirmed presence NCEP-ATP III criteria of metabolic syndrome. The adiponectin level was analyzed by ELISA methods.

Fasting blood glucose, triglyceride, and systolic blood pressure increased significantly (p<0.05) in HFHS group compared to that of NC group. Moreover, after 8 week a significant lower HDL level was observed in HFHS group compared to that of NC group. In addition, HFHS group showed a significantly lower adiponectin level compared to that of NC group.

The combination of low doses of STZ (30mg/kg) and HFHS administration for 8 weeks could induce metabolic syndrome mimicking human criteria of metabolic syndrome.


Keywords


Metabolic syndrome animal model; streptozococin; high fat and high sucrose diet

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References


Alberti, G. (2005). Introduction to the metabolic syndrome. European Heart Journal Supplements, 7 (suppl D), D3-D5.

Aldhahi, W., & Hamdy, O. (2003). Adipokines, inflammation, and the endothelium in diabetes. Current Diabetes Reports, 3 (4), 293-298.

Arshad, S., & Hussain, M. M. (2013). Correlation of Insulin Resistance with Thyroid Profile in Streptozotocin Induced Type 2 Diabetic Rats. Journal of Rawalpindi Medical College (JRMC), 17 (2), 277-280.

Atanasovska, E., Tasic, V., Slaninka-Miceska, M., Alabakovska, S., Zafirov, D., Kostova, E., … Labacevski, N. (2014). Six week follow-up of metabolic effects induced by a high-fat diet and streptozotocin in a rodent model of type 2 diabetes mellitus. Contributions Sec Med Sci, 35 (1), 169-79.

Bantle, J. P. (2009). Dietary Fructose and Metabolic Syndrome and Diabetes. Journal of Nutrition, 139 (6), 1263S-1268S.

Beevers, G., Lip, G. Y., & O’brien, E. (2001). The pathophysiology of hypertension. Bmj, 322 (7291), 912–916.

Bertram, C. E., & Hanson, M. A. (2001). Animal models and programming of the metabolic syndrome Type 2 diabetes. British Medical Bulletin, 60 (1), 103–121.

Cameron, A. J., Shaw, J. E., & Zimmet, P. Z. (2004). The metabolic syndrome: prevalence in worldwide populations. Endocrinology and Metabolism Clinics of North America, 33 (2), 351–375, table of contents.

Cani, P. D., Bibiloni, R., Knauf, C., Waget, A., Neyrinck, A. M., Delzenne, N. M., & Burcelin, R. (2008). Changes in gut microbiota control metabolic endotoxemia-induced inflammation in high-fat diet-induced obesity and diabetes in mice. Diabetes, 57 (6), 1470–1481.

Creely, S. J., McTernan, P. G., Kusminski, C. M., Fisher, ff M., Da Silva, N. F., Khanolkar, M., … Kumar, S. (2007). Lipopolysaccharide activates an innate immune system response in human adipose tissue in obesity and type 2 diabetes. American Journal of Physiology. Endocrinology and Metabolism, 292 (3), E740–747.

Gajda, A. M., Pellizzon, M. A., Ricci, M. R., & Ulman, E. A. (2007). Diet-induced metabolic syndrome in rodent models. Animal Lab News, 74, 775–793.

Grundy, S. M. (2004). Definition of Metabolic Syndrome: Report of the National Heart, Lung, and Blood Institute/American Heart Association Conference on Scientific Issues Related to Definition. Circulation, 109 (3), 433–438.

Ibrahim, A., Natrajan, S., & Ghafoorunissa, R. (2005). Dietary trans-fatty acids alter adipocyte plasma membrane fatty acid composition and insulin sensitivity in rats. Metabolism: Clinical and Experimental, 54 (2), 240–246.

Jang, H.-J., Ridgeway, S. D., & Kim, J. -a. (2013). Effects of the green tea polyphenol epigallocatechin-3-gallate on high-fat diet-induced insulin resistance and endothelial dysfunction. AJP: Endocrinology and Metabolism, 305 (12), E1444–E1451.

Kuate, D., Kengne, A. P. N., Biapa, C. P. N., Azantsa, B. G. K., & Wan Muda, W. A. M. B. (2015). Tetrapleura tetraptera spice attenuates high-carbohydrate, high-fat diet-induced obese and type 2 diabetic rats with metabolic syndrome features. Lipids in Health and Disease, 14.

Lichtenstein, A. H., Jauhiainen, M., McGladdery, S., Ausman, L. M., Jalbert, S. M., Vilella-Bach, M., … Schaefer, E. J. (2001). Impact of hydrogenated fat on high density lipoprotein subfractions and metabolism. Journal of Lipid Research, 42 (4), 597–604.

Novelli, E. L. B., Diniz, Y. S., Galhardi, C. M., Ebaid, G. M. X., Rodrigues, H. G., Mani, F., … Novelli Filho, J. L. V. B. (2007). Anthropometrical parameters and markers of obesity in rats. Laboratory Animals, 41 (1), 111–119.

Suman, R. K., Ray Mohanty, I., Borde, M. K., Maheshwari, U., & Deshmukh, Y. A. (2016). Development of an Experimental Model of Diabetes Co-Existing with Metabolic Syndrome in Rats. Advances in Pharmacological Sciences, 2016, 1–11.

Wang, H. J., Jin, Y. X., Shen, W., Neng, J., Wu, T., Li, Y. J., & Fu, Z. W. (2007). Low dose streptozotocin (STZ) combined with high energy intake can effectively induce type 2 diabetes through altering the related gene expression. Asia Pacific Journal of Clinical Nutrition, 16 (S1), 412–417.

Wong, S. K., Chin, K.-Y., Suhaimi, F. H., Fairus, A., & Ima-Nirwana, S. (2016). Animal models of metabolic syndrome: a review. Nutrition & Metabolism, 13 (1).




DOI: https://doi.org/10.21776/ub.rjls.2017.004.01.10

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