Novel Morphology Development of Murine Bone Marrow-Derived Macrophage Post Differentiation

Renanda Baghaz Dzulhamdhani Surya Putra, Sri Andayani, Ko-Tung Chang

Abstract


Bone marrow-derived macrophages (BMDM) are primary macrophage cells,widely used for studying macrophage function. However, the development of BMDM after differentiation notable still unknown. This research aimed to observe the development of BMDM post differentiation with M-CSF, also distinguished the differences. In present study we observed morphology the development of BMDM under microscope and examined the gene transcript level expression by quantitative real-time PCR. Post differentiation BMDM at day 8-13 no proliferation and spindle-like cell shape was observed. In contrast, at day 14 the morphology of cell become round shape. Interestingly, the cells started to proliferate was observed at day 14 to day 20. Similar tendency both of RAW 264.7 and BMDM at day 20 were observed. Concisely, both of them had a round shape, high proliferation, and the adherence was similar.In gene transcript level expression BMDM at day 20 has higher M2 phenotype by suppressing gene transcript level of TNF-α, IRF5 and increasing gene transcript level of IRF4 compared than BMDM at day 7. Our results show that BMDM in long term time was altered the morphology and has different phenotype in biological functions,providing clues to guide their further experimental or therapeutic use.

Keywords


Monocyte; BMDM; macrophage polarization

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DOI: https://doi.org/10.21776/ub.rjls.2018.005.01.1

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